Abstract: Objective　To explore the clinical value of the analysis of lymphocyte subsets and immunoglobulin in children with Kawasaki disease (KD). Methods　Three hundred and eighty-eight children with KD, 160 children with infectious febrile disease and 85 normal children for well child visit were enrolled. The absolute value and percentage of T lymphocyte subsets, natural killer cells (NK cells) and B lymphocytes were detected by flow cytometry. The serum levels of IgG, IgM, IgA, and complement C3, C4 were assessed by velocity scatter turbidimetry. Results　Compared with infectious febrile children and normal control children, the levels of B cells, complements C3 and ratio of CD4/CD8 cells were significantly increased, the percentage of CD8+T cells and NK cells significantly decreased, and the absolute values of CD3+T cells significantly increased in children with KD (all P < 0.05). Conclusions　Lymphocytes subsets and complements might be used as laboratory index to identify KD and understand the pathogenesis of KD.